mouse coronary artery endothelial cell mcaec culture primary mouse coronary arterial endothelial cells  (Celprogen Inc)

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet: The distance between the endothelial cells in the insert and the cardiomyocytes at the bottom of the well can be varied by using different inserts. All three types of inserts used here have the same pore size of 0.4 μm. The only difference among them is the insert-to-base height, which allows the distances between the two co-cultured cell layers to be 0.5 (A), 1.0 (B) and 2.0 mm (C), respectively.

Article Snippet: 29 , Mouse Primary Coronary Artery Endothelial Cells (ECs) , Cell Biologics Inc , C57–6093 , Isolated from coronary artery of C57BL/6 mice.

Techniques: Pore Size, Cell Culture

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 29 , Mouse Primary Coronary Artery Endothelial Cells (ECs) , Cell Biologics Inc , C57–6093 , Isolated from coronary artery of C57BL/6 mice.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Basic research in cardiology

Article Title: Effect of PAR2 in regulating TNF- α and NAD(P)H oxidase in coronary arterioles in type 2 diabetic mice

doi: 10.1007/s00395-010-0129-9

Figure Lengend Snippet: a Dual fluorescence combining PAR2 with markers for endothelial cells [von Willebrand factor (vWF)], vascular smooth muscle cells (α-actin) and macrophages (CD68). A, B and C, dual labeling of PAR2 (red) and vWF (green) in control mouse heart tissue. E, F and G, dual labeling of PAR2 (red) and vWF (green) in db/db mouse heart tissue. The blue arrows in C and G show the colocalization of PAR2 and endothelial cells (yellow) in control and db/db mice. The inserts in C (D) and the inserts in G (H, I) show the higher magnification of the colocalization pointed by blue arrows in C and G. J, K and L, dual labeling of PAR2 (red) and α-actin (green) in control mice heart tissue. N, O and P, dual labeling of PAR2 (red) and α-actin (green) in db/db mice heart tissue. The pink arrows in L and P show the colocalization of PAR2 and vascular smooth muscle cells (yellow) in control and db/db mice. The inserts in L (M) and the inserts in P (Q) show the higher magnification of the colocalization pointed by pink arrows in L and P. R, S and T, dual labeling of PAR2 (red) and marker of macrophage in db/db mouse heart tissue. The brown arrows in T show the specific CD68 staining with absence of PAR2 staining. U and V, negative control: the purple arrows show the absence of staining in vessels without primary antibodies. W shows nuclear staining with DAPI (blue) in db/db mouse heart tissue. The immunostaining results suggest that in the heart of type 2 diabetic mice, PAR2 is expressed in endothelial cells and vascular smooth muscle cells, but not in macrophages. Magnification, ×40. Data shown are representative of four separate experiments. b PAR2 is expressed in mouse coronary arterial endothelial cells and it was significantly increased with the presence of TNF-α. However, there was no TNF-α concentration-dependent (1, 3, or 10 ng/mL) increase in PAR2 expression, n = 3. Data were expressed as mean ± SEM. * P < 0.05 versus WT

Article Snippet: Primary mouse coronary arterial endothelial cells (Celprogen, San Pedro, CA, USA) were maintained in culture in the presence or absence of recombinant TNF- α (R&D: control, 1, 3 and 10 ng/mL for 24 h).

Techniques: Fluorescence, Labeling, Marker, Staining, Negative Control, Immunostaining, Concentration Assay, Expressing